Please use this identifier to cite or link to this item:
https://ah.lib.nccu.edu.tw/handle/140.119/65233
題名: | A gene delivery system for insect cells mediated by arginine-rich cell-penetrating peptides | 作者: | Chen, Yung-Jen ; Liu, Betty Revon ; Dai, Yun-Hao ; Lee, Cheng-Yi ; Chan, Ming-Huan ; Chen, Hwei-Hsien ; Chiang, Huey-Jenn ; Lee, Han-Jung 詹銘煥 |
貢獻者: | 神科所 | 關鍵詞: | Cell-penetrating peptide (CPP); Cellular internalization; Polyarginine; Protein transduction; Spodoptera frugiperda; Transgenic insects | 日期: | 2012 | 上傳時間: | 9-Apr-2014 | 摘要: | Most bioactive macromolecules, such as protein, DNA and RNA, basically cannot permeate into cells freely from outside the plasma membrane. Cell-penetrating peptides (CPPs) are a group of short peptides that possess the ability to traverse the cell membrane and have been considered as candidates for mediating gene and drug delivery into living cells. In this study, we demonstrate that three arginine-rich CPPs (SR9, HR9 and PR9) are able to form stable complexes with plasmid DNA and deliver DNA into insect Sf9 cells in a noncovalent manner. The transferred plasmid DNA containing enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) coding regions could be expressed in cells functionally assayed at both the protein and RNA levels. Furthermore, treatment of cells with CPPs and CPP/DNA complexes resulted in a viability of 84–93% indicating these CPPs are not cytotoxic. These results suggest that arginine-rich CPPs appear to be a promising tool for insect transgenesis | 關聯: | Gene,493(2), 201-210 | 資料來源: | http://dx.doi.org/10.1016/j.gene.2011.11.060 | 資料類型: | article |
Appears in Collections: | 期刊論文 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
201210.pdf | 2.09 MB | Adobe PDF2 | View/Open |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.