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https://ah.lib.nccu.edu.tw/handle/140.119/101131| 題名: | 在腎上腺摘除模式下觀察微小膠細胞與成體神經前驅細胞之間的關係 Microglia regulation of adult neural progenitor cells |
作者: | 蔡欣學 | 貢獻者: | 賴桂珍 蔡欣學 |
關鍵詞: | 微小膠細胞 | 日期: | 2016 | 上傳時間: | 1-Sep-2016 | 摘要: | 微小膠細胞(microglia)為中樞神經系統中主要免疫功能的神經膠細胞，並且具有吞噬的功能用來移除生病或邁向死亡的細胞。但是近年來越來越多實驗發現微小膠細胞也會調控神經細胞的增生以及分化，甚至可能與調控神經新生有關係。\n在腎上腺摘除(ADX)之後發現到齒狀迴(dentate gyrus)中的DGCs死亡以及activated microlgia數量增加，而且這些microglia與NPCs培養後會影響NPCs的分化，因此我們想要利用此模式進一步來了解同樣在hippocampus中的cornus ammonis(CA)以及掌管我們認知行為的PFC腦區在腎上腺摘除之後的microglia否會影響NPCs。實驗結果顯示在ADX後microglia與NPCs共養後，會影響NPCs的cell number以及neurite length。而在CA區取出的ADX microglia-condition medium培養NPCs後發現到會減少NPCs的cell number。從以上可以得知在ADX後的microglia的確改變了自身功能進而影響到了NPCs的分化以及生長，而且來自PFC腦區的Microglia與來自CA腦區的Microglia對於NPCs的影響也不同。 | 參考文獻: | Aarum, J., Sandberg, K., Haeberlein, S. L., & Persson, M. A. (2003). Migration and differentiation of neural precursor cells can be directed by microglia. Proc Natl Acad Sci U S A, 100(26), 15983-15988. doi: 10.1073/pnas.2237050100\nAguzzi, A., Barres, B. A., & Bennett, M. L. (2013). Microglia: scapegoat, saboteur, or something else? 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資料來源: | http://thesis.lib.nccu.edu.tw/record/#G0100754009 | 資料類型: | thesis |
| Appears in Collections: | 學位論文 |
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