Please use this identifier to cite or link to this item:
https://ah.lib.nccu.edu.tw/handle/140.119/64582
DC Field | Value | Language |
---|---|---|
dc.contributor | 風管系 | en_US |
dc.creator | Chiu, Sung-Kay ; Hsieh, Ming-Hua ; Tzeng, Chi-Meng | en_US |
dc.creator | 謝明華;曾驥孟 | zh_TW |
dc.date | 2011-03 | en_US |
dc.date.accessioned | 2014-03-11T09:38:43Z | - |
dc.date.available | 2014-03-11T09:38:43Z | - |
dc.date.issued | 2014-03-11T09:38:43Z | - |
dc.identifier.uri | http://nccur.lib.nccu.edu.tw/handle/140.119/64582 | - |
dc.description.abstract | By taking advantage of the power of comparative genomics, we devised an algorithm, Unique Marker Finder (U-MarFin), to generate a collection of unique DNA sequences from a target organism. The whole target genome is partitioned into a scoring pool of less 4000 base-pair fragments, which are then subjected to elimination of homologous sequences in other bacterial genomes by BLAST alignment, and looked for all open reading frames as they may be applied as unique markers. Through regular, nested, multiplex and real time PCR and microarray technology, we empirically demonstrated that the sequences discovered were highly specific to the species that they are derived from, and they can serve as molecular biomarkers for diagnostic purpose. | en_US |
dc.format.extent | 107 bytes | - |
dc.format.mimetype | text/html | - |
dc.language.iso | en_US | - |
dc.relation | International Journal of Bioinformatics Research and Applications, 7(1), 24-42 | en_US |
dc.subject | comparative genomics; subtraction; diagnostic markers;micro-array; real-time PCR. | en_US |
dc.title | Unique marker finder algorithm generates molecular diagnostic markers | en_US |
dc.type | article | en |
dc.identifier.doi | 10.1504/IJBRA.2011.039168 | en_US |
dc.doi.uri | http://dx.doi.org/10.1504/IJBRA.2011.039168 | en_US |
item.languageiso639-1 | en_US | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.openairetype | article | - |
item.grantfulltext | restricted | - |
item.cerifentitytype | Publications | - |
Appears in Collections: | 期刊論文 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
index.html | 107 B | HTML2 | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.