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Title: Identification of plant vacuolar transporters mediating phosphate storage
Authors: 蔡尚岳
Liu, Tzu-Yin;Huang, Teng-Kuei;Yang, Shu-Yi;Hong, Yu-Ting;Huang, Sheng-Min;Wang, Fu-Nien
Contributors: 應物所
Date: 2016-03
Issue Date: 2016-05-30 15:22:33 (UTC+8)
Abstract: Plant vacuoles serve as the primary intracellular compartments for inorganic phosphate (Pi) storage. Passage of Pi across vacuolar membranes plays a critical role in buffering the cytoplasmic Pi level against fluctuations of external Pi and metabolic activities. Here we demonstrate that the SPX-MFS proteins, designated as PHOSPHATE TRANSPORTER 5 family (PHT5), also named Vacuolar Phosphate Transporter (VPT), function as vacuolar Pi transporters. Based on 31P-magnetic resonance spectroscopy analysis, Arabidopsis pht5;1 loss-of-function mutants accumulate less Pi and exhibit a lower vacuolar-to-cytoplasmic Pi ratio than controls. Conversely, overexpression of PHT5 leads to massive Pi sequestration into vacuoles and altered regulation of Pi starvation-responsive genes. Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles. Our findings show that a group of Pi transporters in vacuolar membranes regulate cytoplasmic Pi homeostasis and are required for fitness and plant growth.
Relation: Nature Communications, Vol.31, No.7, pp.11095
Data Type: article
DOI 連結:
Appears in Collections:[應用物理研究所 ] 期刊論文

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