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題名: | Identification of plant vacuolar transporters mediating phosphate storage | 作者: | 蔡尚岳 Liu, Tzu-Yin;Huang, Teng-Kuei;Yang, Shu-Yi;Hong, Yu-Ting;Huang, Sheng-Min;Wang, Fu-Nien |
貢獻者: | 應物所 | 日期: | 三月-2016 | 上傳時間: | 30-五月-2016 | 摘要: | Plant vacuoles serve as the primary intracellular compartments for inorganic phosphate (Pi) storage. Passage of Pi across vacuolar membranes plays a critical role in buffering the cytoplasmic Pi level against fluctuations of external Pi and metabolic activities. Here we demonstrate that the SPX-MFS proteins, designated as PHOSPHATE TRANSPORTER 5 family (PHT5), also named Vacuolar Phosphate Transporter (VPT), function as vacuolar Pi transporters. Based on 31P-magnetic resonance spectroscopy analysis, Arabidopsis pht5;1 loss-of-function mutants accumulate less Pi and exhibit a lower vacuolar-to-cytoplasmic Pi ratio than controls. Conversely, overexpression of PHT5 leads to massive Pi sequestration into vacuoles and altered regulation of Pi starvation-responsive genes. Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles. Our findings show that a group of Pi transporters in vacuolar membranes regulate cytoplasmic Pi homeostasis and are required for fitness and plant growth. | 關聯: | Nature Communications, Vol.31, No.7, pp.11095 | 資料類型: | article | DOI: | http://dx.doi.org/10.1038/ncomms11095 |
Appears in Collections: | 期刊論文 |
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