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題名 Polycomb-Dependent Chromatin Looping Contributes to Gene Silencing during Drosophila Development
作者 Ogiyama, Yuki
Schuettengruber, Bernd
Papadopoulos, Giorgio L.
張家銘
Chang, Jia-Ming
Cavalli, Giacomo
貢獻者 資訊科學系
關鍵詞 3D genome; TADs; chromosome organization; chromatin; Hi-C; Drosophila embryogenesis; polycom; blooping interactions
日期 2018-07
上傳時間 24-七月-2018 17:29:42 (UTC+8)
摘要 Interphase chromatin is organized into topologically associating domains (TADs). Within TADs, chromatin looping interactions are formed between DNA regulatory elements, but their functional importance for the establishment of the 3D genome organization and gene regulation during development is unclear. Using high-resolution Hi-C experiments, we analyze higher order 3D chromatin organization during Drosophila embryogenesis and identify active and repressive chromatin loops that are established with different kinetics and depend on distinct factors: Zelda-dependent active loops are formed before the midblastula transition between transcribed genes over long distances. Repressive loops within polycomb domains are formed after the midblastula transition between polycomb response elements by the action of GAGA factor and polycomb proteins. Perturbation of PRE function by CRISPR/Cas9 genome engineering affects polycomb domain formation and destabilizes polycomb-mediated silencing. Preventing loop formation without removal of polycomb components also decreases silencing efficiency, suggesting that chromatin architecture can play instructive roles in gene regulation during development.
關聯 Molecular Cell, Volume 71, Issue 1, Pages 73-88.e5
資料類型 article
DOI https://doi.org/10.1016/j.molcel.2018.05.032
dc.contributor 資訊科學系
dc.creator (作者) Ogiyama, Yukien_US
dc.creator (作者) Schuettengruber, Bernden_US
dc.creator (作者) Papadopoulos, Giorgio L.en_US
dc.creator (作者) 張家銘zh_TW
dc.creator (作者) Chang, Jia-Mingen_US
dc.creator (作者) Cavalli, Giacomoen_US
dc.date (日期) 2018-07
dc.date.accessioned 24-七月-2018 17:29:42 (UTC+8)-
dc.date.available 24-七月-2018 17:29:42 (UTC+8)-
dc.date.issued (上傳時間) 24-七月-2018 17:29:42 (UTC+8)-
dc.identifier.uri (URI) http://nccur.lib.nccu.edu.tw/handle/140.119/118879-
dc.description.abstract (摘要) Interphase chromatin is organized into topologically associating domains (TADs). Within TADs, chromatin looping interactions are formed between DNA regulatory elements, but their functional importance for the establishment of the 3D genome organization and gene regulation during development is unclear. Using high-resolution Hi-C experiments, we analyze higher order 3D chromatin organization during Drosophila embryogenesis and identify active and repressive chromatin loops that are established with different kinetics and depend on distinct factors: Zelda-dependent active loops are formed before the midblastula transition between transcribed genes over long distances. Repressive loops within polycomb domains are formed after the midblastula transition between polycomb response elements by the action of GAGA factor and polycomb proteins. Perturbation of PRE function by CRISPR/Cas9 genome engineering affects polycomb domain formation and destabilizes polycomb-mediated silencing. Preventing loop formation without removal of polycomb components also decreases silencing efficiency, suggesting that chromatin architecture can play instructive roles in gene regulation during development.en_US
dc.format.extent 6053584 bytes-
dc.format.mimetype application/pdf-
dc.relation (關聯) Molecular Cell, Volume 71, Issue 1, Pages 73-88.e5
dc.subject (關鍵詞) 3D genome; TADs; chromosome organization; chromatin; Hi-C; Drosophila embryogenesis; polycom; blooping interactionsen_US
dc.title (題名) Polycomb-Dependent Chromatin Looping Contributes to Gene Silencing during Drosophila Developmenten_US
dc.type (資料類型) article
dc.identifier.doi (DOI) 10.1016/j.molcel.2018.05.032
dc.doi.uri (DOI) https://doi.org/10.1016/j.molcel.2018.05.032