Please use this identifier to cite or link to this item:
https://ah.lib.nccu.edu.tw/handle/140.119/96994
DC Field | Value | Language |
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dc.contributor | 應物所 | |
dc.creator | 蔡尚岳 | zh_TW |
dc.creator | Liu, Tzu-Yin;Huang, Teng-Kuei;Yang, Shu-Yi;Hong, Yu-Ting;Huang, Sheng-Min;Wang, Fu-Nien | |
dc.date | 2016-03 | |
dc.date.accessioned | 2016-05-30T07:22:33Z | - |
dc.date.available | 2016-05-30T07:22:33Z | - |
dc.date.issued | 2016-05-30T07:22:33Z | - |
dc.identifier.uri | http://nccur.lib.nccu.edu.tw/handle/140.119/96994 | - |
dc.description.abstract | Plant vacuoles serve as the primary intracellular compartments for inorganic phosphate (Pi) storage. Passage of Pi across vacuolar membranes plays a critical role in buffering the cytoplasmic Pi level against fluctuations of external Pi and metabolic activities. Here we demonstrate that the SPX-MFS proteins, designated as PHOSPHATE TRANSPORTER 5 family (PHT5), also named Vacuolar Phosphate Transporter (VPT), function as vacuolar Pi transporters. Based on 31P-magnetic resonance spectroscopy analysis, Arabidopsis pht5;1 loss-of-function mutants accumulate less Pi and exhibit a lower vacuolar-to-cytoplasmic Pi ratio than controls. Conversely, overexpression of PHT5 leads to massive Pi sequestration into vacuoles and altered regulation of Pi starvation-responsive genes. Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles. Our findings show that a group of Pi transporters in vacuolar membranes regulate cytoplasmic Pi homeostasis and are required for fitness and plant growth. | |
dc.format.extent | 1267030 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.relation | Nature Communications, Vol.31, No.7, pp.11095 | |
dc.title | Identification of plant vacuolar transporters mediating phosphate storage | |
dc.type | article | |
dc.identifier.doi | 10.1038/ncomms11095 | |
dc.doi.uri | http://dx.doi.org/10.1038/ncomms11095 | |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.grantfulltext | restricted | - |
Appears in Collections: | 期刊論文 |
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File | Description | Size | Format | |
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410136.pdf | 1.24 MB | Adobe PDF2 | View/Open |
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