| dc.contributor | 應物所 | |
| dc.creator (作者) | 蔡尚岳 | zh_TW |
| dc.creator (作者) | Liu, Tzu-Yin;Huang, Teng-Kuei;Yang, Shu-Yi;Hong, Yu-Ting;Huang, Sheng-Min;Wang, Fu-Nien | |
| dc.date (日期) | 2016-03 | |
| dc.date.accessioned | 30-May-2016 15:22:33 (UTC+8) | - |
| dc.date.available | 30-May-2016 15:22:33 (UTC+8) | - |
| dc.date.issued (上傳時間) | 30-May-2016 15:22:33 (UTC+8) | - |
| dc.identifier.uri (URI) | http://nccur.lib.nccu.edu.tw/handle/140.119/96994 | - |
| dc.description.abstract (摘要) | Plant vacuoles serve as the primary intracellular compartments for inorganic phosphate (Pi) storage. Passage of Pi across vacuolar membranes plays a critical role in buffering the cytoplasmic Pi level against fluctuations of external Pi and metabolic activities. Here we demonstrate that the SPX-MFS proteins, designated as PHOSPHATE TRANSPORTER 5 family (PHT5), also named Vacuolar Phosphate Transporter (VPT), function as vacuolar Pi transporters. Based on 31P-magnetic resonance spectroscopy analysis, Arabidopsis pht5;1 loss-of-function mutants accumulate less Pi and exhibit a lower vacuolar-to-cytoplasmic Pi ratio than controls. Conversely, overexpression of PHT5 leads to massive Pi sequestration into vacuoles and altered regulation of Pi starvation-responsive genes. Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles. Our findings show that a group of Pi transporters in vacuolar membranes regulate cytoplasmic Pi homeostasis and are required for fitness and plant growth. | |
| dc.format.extent | 1267030 bytes | - |
| dc.format.mimetype | application/pdf | - |
| dc.relation (關聯) | Nature Communications, Vol.31, No.7, pp.11095 | |
| dc.title (題名) | Identification of plant vacuolar transporters mediating phosphate storage | |
| dc.type (資料類型) | article | |
| dc.identifier.doi (DOI) | 10.1038/ncomms11095 | |
| dc.doi.uri (DOI) | http://dx.doi.org/10.1038/ncomms11095 | |
