| dc.contributor | 資科系 | zh_Tw |
| dc.creator (作者) | 張家銘 | zh_TW |
| dc.creator (作者) | Cattoni, Diego I.;González, Inma;Chang, Jia-Ming;Sexton, Thomas;Marc, A. Marti-Renom;Bantignies, Frédéric | en_US |
| dc.date (日期) | 2017-11 | |
| dc.date.accessioned | 17-Apr-2018 15:49:36 (UTC+8) | - |
| dc.date.available | 17-Apr-2018 15:49:36 (UTC+8) | - |
| dc.date.issued (上傳時間) | 17-Apr-2018 15:49:36 (UTC+8) | - |
| dc.identifier.uri (URI) | http://nccur.lib.nccu.edu.tw/handle/140.119/116885 | - |
| dc.description.abstract (摘要) | At the kilo- to mega-base pair scales, eukaryotic genomes are partitioned into self-interacting modules or topologically associated domains (TADs) that associate to form nuclear compartments. Here, we combined high-content super-resolution microscopies with state-of-the-art DNA labeling methods to reveal the variability in the multiscale organization of the Drosophila genome. We found that association frequencies within TADs and between TAD borders are below ~10%, independently of TAD size, epigenetic state, or cell type. Critically, despite this large heterogeneity, we were able to visualize nanometer-sized epigenetic domains at the single-cell level. In addition, absolute contact frequencies within and between TADs were to a large extent defined by genomic distance, higher-order chromosome architecture, and epigenetic identity. We propose that TADs and compartments are organized by multiple, small frequency, yet specific interactions that are regulated by epigenetics and transcriptional state. | en_US |
| dc.format.extent | 4203397 bytes | - |
| dc.format.mimetype | application/pdf | - |
| dc.relation (關聯) | Nature communications | |
| dc.title (題名) | Single-cell absolute contact probability detection reveals chromosomes are organized by multiple low-frequency yet specific interactions | en_US |
| dc.type (資料類型) | article | |
| dc.identifier.doi (DOI) | 10.1101/159814 | |
| dc.doi.uri (DOI) | https://doi.org/10.1101/159814 | |
