Publications-Periodical Articles

Article View/Open

Publication Export

Google ScholarTM

NCCU Library

Citation Infomation

  • Loading...
    SCOPUS®38

Related Publications in TAIR

TitleNeurotensin-Conjugated Reduced Graphene Oxide with Multi-Stage Near-Infrared-Triggered Synergic Targeted Neuron Gene Transfection In Vitro and In Vivo for Neurodegenerative Disease Therapy
CreatorHsieh, Tsung‐Ying;Huang, Wei‐Chen;Kang, Yi‐Da;Chu, Chao‐Yi;Liao, Wen‐Lin;Chen, You‐Yin
廖文霖
Liao, Wen‐Lin
Contributor神科所
Key Wordscentral nervous system; gene transfection; graphene oxide; laser photothermal treatment; nanoparticles
Date2016-12
Date Issued17-Sep-2018 18:00:55 (UTC+8)
SummaryDelivery efficiency with gene transfection is a pivotal point in achieving maximized therapeutic efficacy and has been an important challenge with central nervous system (CNS) diseases. In this study, neurotensin (NT, a neuro-specific peptide)-conjugated polyethylenimine (PEI)-modified reduced graphene oxide (rGO) nanoparticles with precisely controlled two-stage near-infrared (NIR)-laser photothermal treatment to enhance the ability to target neurons and achieve high gene transfection in neurons. First-stage NIR laser irradiation on the cells with nanoparticles attached on the surface can increase the permeability of the cell membrane, resulting in an apparent increase in cellular uptake compared to untreated cells. In addition, second-stage NIR laser irradiation on the cells with nanoparticles inside can further induce endo/lysosomal cavitation, which not only helps nanoparticles escape from endo/lysosomes but also prevents plasmid DNA (pDNA) from being digested by DNase I. At least double pDNA amount can be released from rGO-PEI-NT/pDNA under NIR laser trigger release compared to natural release. Moreover, in vitro differentiated PC-12 cell and in vivo mice (C57BL/6) brain transfection experiments have demonstrated the highest transfection efficiency occurring when NT modification is combined with external multi-stage stimuli-responsive NIR laser treatment. The combination of neuro-specific targeting peptide and external NIR-laser-triggered aid provides a nanoplatform for gene therapy in CNS diseases.
RelationAdvanced Healthcare Materials, Volume5, Issue23, Pages 3016-3026
PMID: 27805786
Typearticle
DOI https://doi.org/10.1002/adhm.201600647
dc.contributor 神科所
dc.creator (作者) Hsieh, Tsung‐Ying;Huang, Wei‐Chen;Kang, Yi‐Da;Chu, Chao‐Yi;Liao, Wen‐Lin;Chen, You‐Yinen_US
dc.creator (作者) 廖文霖zh_TW
dc.creator (作者) Liao, Wen‐Linen_US
dc.date (日期) 2016-12
dc.date.accessioned 17-Sep-2018 18:00:55 (UTC+8)-
dc.date.available 17-Sep-2018 18:00:55 (UTC+8)-
dc.date.issued (上傳時間) 17-Sep-2018 18:00:55 (UTC+8)-
dc.identifier.uri (URI) http://nccur.lib.nccu.edu.tw/handle/140.119/120100-
dc.description.abstract (摘要) Delivery efficiency with gene transfection is a pivotal point in achieving maximized therapeutic efficacy and has been an important challenge with central nervous system (CNS) diseases. In this study, neurotensin (NT, a neuro-specific peptide)-conjugated polyethylenimine (PEI)-modified reduced graphene oxide (rGO) nanoparticles with precisely controlled two-stage near-infrared (NIR)-laser photothermal treatment to enhance the ability to target neurons and achieve high gene transfection in neurons. First-stage NIR laser irradiation on the cells with nanoparticles attached on the surface can increase the permeability of the cell membrane, resulting in an apparent increase in cellular uptake compared to untreated cells. In addition, second-stage NIR laser irradiation on the cells with nanoparticles inside can further induce endo/lysosomal cavitation, which not only helps nanoparticles escape from endo/lysosomes but also prevents plasmid DNA (pDNA) from being digested by DNase I. At least double pDNA amount can be released from rGO-PEI-NT/pDNA under NIR laser trigger release compared to natural release. Moreover, in vitro differentiated PC-12 cell and in vivo mice (C57BL/6) brain transfection experiments have demonstrated the highest transfection efficiency occurring when NT modification is combined with external multi-stage stimuli-responsive NIR laser treatment. The combination of neuro-specific targeting peptide and external NIR-laser-triggered aid provides a nanoplatform for gene therapy in CNS diseases.en_US
dc.format.extent 108 bytes-
dc.format.mimetype text/html-
dc.relation (關聯) Advanced Healthcare Materials, Volume5, Issue23, Pages 3016-3026
dc.relation (關聯) PMID: 27805786
dc.subject (關鍵詞) central nervous system; gene transfection; graphene oxide; laser photothermal treatment; nanoparticlesen_US
dc.title (題名) Neurotensin-Conjugated Reduced Graphene Oxide with Multi-Stage Near-Infrared-Triggered Synergic Targeted Neuron Gene Transfection In Vitro and In Vivo for Neurodegenerative Disease Therapyen_US
dc.type (資料類型) article
dc.identifier.doi (DOI) 10.1002/adhm.201600647
dc.doi.uri (DOI) https://doi.org/10.1002/adhm.201600647