1. NCCU Academic Hub
  2. 學術產出
  3. College of Science
  4. Periodical Articles
  5. Item

學術產出-Periodical Articles

Article View/Open

Publication Export

Google ScholarTM

政大圖書館

Citation Infomation

題名 Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor: Honokiol and Muscarinic Signaling
作者 詹銘煥
Chan,Ming-Huan
貢獻者 神科所
關鍵詞 Calcium;Proteins;Rodents;Experiments;Life sciences;Binding sites;Neurosciences;Cell culture;Cytotoxicity
日期 2013.02
上傳時間 3-Dec-2013 18:20:20 (UTC+8)
摘要 Background: Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees and present in Asian herbal teas, has a unique array of pharmacological actions, including the inhibition of multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by human M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Receptor binding was determined in radiolabelled ligand binding assays; changes in intracellular calcium concentrations were determined using a fura-2 ratiometric imaging protocol; cytotoxicity was determined using a dye reduction assay. Results: Honokiol had a potent (EC50 ≈ 5 μmol/l) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and partially reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding. Conclusions: It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties.
關聯 Journal of Biomedical Science, 20(1), 11
資料類型 article
DOI http://dx.doi.org/10.1186/1423-0127-20-11
dc.contributor 神科所en_US
dc.creator (作者) 詹銘煥zh_TW
dc.creator (作者) Chan,Ming-Huanen_US
dc.date (日期) 2013.02en_US
dc.date.accessioned 3-Dec-2013 18:20:20 (UTC+8)-
dc.date.available 3-Dec-2013 18:20:20 (UTC+8)-
dc.date.issued (上傳時間) 3-Dec-2013 18:20:20 (UTC+8)-
dc.identifier.uri (URI) http://nccur.lib.nccu.edu.tw/handle/140.119/62103-
dc.description.abstract (摘要) Background: Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees and present in Asian herbal teas, has a unique array of pharmacological actions, including the inhibition of multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by human M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Receptor binding was determined in radiolabelled ligand binding assays; changes in intracellular calcium concentrations were determined using a fura-2 ratiometric imaging protocol; cytotoxicity was determined using a dye reduction assay. Results: Honokiol had a potent (EC50 ≈ 5 μmol/l) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and partially reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding. Conclusions: It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties.-
dc.format.extent 1820253 bytes-
dc.format.mimetype application/pdf-
dc.language.iso en_US-
dc.relation (關聯) Journal of Biomedical Science, 20(1), 11en_US
dc.subject (關鍵詞) Calcium;Proteins;Rodents;Experiments;Life sciences;Binding sites;Neurosciences;Cell culture;Cytotoxicityen_US
dc.title (題名) Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor: Honokiol and Muscarinic Signalingen_US
dc.type (資料類型) articleen
dc.identifier.doi (DOI) 10.1186/1423-0127-20-11en_US
dc.doi.uri (DOI) http://dx.doi.org/10.1186/1423-0127-20-11en_US