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題名 Astrgaloside IV increases MMP-2 mRNA and protein expression in human lung cancer A549 cells. 作者 Su, Chin-Cheng ; Chiou, Tsrong-Laang ; Chan, Ming-Huan ; Lin, Jaung-Geng
詹銘煥貢獻者 神科所 日期 2009.01 上傳時間 9-Apr-2014 17:43:57 (UTC+8) 摘要 Lung cancer is the leading cause of cancer-related death in Taiwan. In the clinical treatment of lung cancer patients in Asia, an Astragalus-based herbal mixture is commonly used in conjunction with chemotherapy. The principal component of Astragalus (also known as Huang-qi) is Astragaloside IV. The Astragaloside IV marker has been qualified for the phytochemicals of Astragalus. The recurrence and metastasis of lung cancer are positively correlated with matrix metalloproteinase-2 (MMP-2) mRNA and protein expression. This study examined the effects of Astragaloside IV on the mRNA and protein expression of MMP-2 in lung cancer A549 cells by RT-PCR and Western blotting. The cytotoxicity of Astragaloside IV in these cells was determined using CellTiter 96® AQueous One Solution Reagent and the MTT assay. A549 cells were treated for different durations with Astragaloside IV concentrations of 10, 20, 50 and 100 ng/ml. The respective proliferation rates per amount (relative to the control) were as follows: 24 h, 96.85±1.12, 95.63±0.83, 93.92±0.84, 95.27±0.57%; 48 h, 98.86±1.56, 95.71±0.59, 94.09±0.68, 93.44±0.5%; 72 h, 99.48±0.16, 95.60±0.48, 95.23±0.67, 94.72±1.12%. MMP-2 mRNA expression as well as vascular endothelial growth factor mRNA expression were upregulated at concentrations of 10, 20 and 50 ng/ml. Additionally, protein expression of MMP-2 was increased at concentrations of 10, 20 and 30 µg/ml after 24 h of treatment. These results indicate that Astragaloside IV upregulates MMP-2 mRNA and protein expression in A549 cells, and therefore that it may increase recurrence and metastatic rates in lung cancer. This issue should be further examined in the clinical setting. 關聯 Molecular Medicine Report,2(1), 107-113 資料類型 article DOI http://dx.doi.org/10.3892/mmr_00000070 dc.contributor 神科所 en_US dc.creator (作者) Su, Chin-Cheng ; Chiou, Tsrong-Laang ; Chan, Ming-Huan ; Lin, Jaung-Geng en_US dc.creator (作者) 詹銘煥 zh_TW dc.date (日期) 2009.01 en_US dc.date.accessioned 9-Apr-2014 17:43:57 (UTC+8) - dc.date.available 9-Apr-2014 17:43:57 (UTC+8) - dc.date.issued (上傳時間) 9-Apr-2014 17:43:57 (UTC+8) - dc.identifier.uri (URI) http://nccur.lib.nccu.edu.tw/handle/140.119/65235 - dc.description.abstract (摘要) Lung cancer is the leading cause of cancer-related death in Taiwan. In the clinical treatment of lung cancer patients in Asia, an Astragalus-based herbal mixture is commonly used in conjunction with chemotherapy. The principal component of Astragalus (also known as Huang-qi) is Astragaloside IV. The Astragaloside IV marker has been qualified for the phytochemicals of Astragalus. The recurrence and metastasis of lung cancer are positively correlated with matrix metalloproteinase-2 (MMP-2) mRNA and protein expression. This study examined the effects of Astragaloside IV on the mRNA and protein expression of MMP-2 in lung cancer A549 cells by RT-PCR and Western blotting. The cytotoxicity of Astragaloside IV in these cells was determined using CellTiter 96® AQueous One Solution Reagent and the MTT assay. A549 cells were treated for different durations with Astragaloside IV concentrations of 10, 20, 50 and 100 ng/ml. The respective proliferation rates per amount (relative to the control) were as follows: 24 h, 96.85±1.12, 95.63±0.83, 93.92±0.84, 95.27±0.57%; 48 h, 98.86±1.56, 95.71±0.59, 94.09±0.68, 93.44±0.5%; 72 h, 99.48±0.16, 95.60±0.48, 95.23±0.67, 94.72±1.12%. MMP-2 mRNA expression as well as vascular endothelial growth factor mRNA expression were upregulated at concentrations of 10, 20 and 50 ng/ml. Additionally, protein expression of MMP-2 was increased at concentrations of 10, 20 and 30 µg/ml after 24 h of treatment. These results indicate that Astragaloside IV upregulates MMP-2 mRNA and protein expression in A549 cells, and therefore that it may increase recurrence and metastatic rates in lung cancer. This issue should be further examined in the clinical setting. en_US dc.format.extent 563920 bytes - dc.format.mimetype application/pdf - dc.language.iso en_US - dc.relation (關聯) Molecular Medicine Report,2(1), 107-113 en_US dc.title (題名) Astrgaloside IV increases MMP-2 mRNA and protein expression in human lung cancer A549 cells. en_US dc.type (資料類型) article en dc.identifier.doi (DOI) 10.3892/mmr_00000070 en_US dc.doi.uri (DOI) http://dx.doi.org/10.3892/mmr_00000070 en_US
