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| Title | RARbeta isoform-specific regulation of DARPP-32 gene expression: An ectopic expression study in the developing telencephalon |
| Creator | 廖文霖 Liao, Wen-Lin ; Liu, Fu-Chin |
| Contributor | 神科所 |
| Key Words | dopamine;retinoic acid;RXR;striatum |
| Date | 2005.01 |
| Date Issued | 23-Sep-2014 12:27:02 (UTC+8) |
| Summary | Dopamine and adenosine 3`:5`-monophosphate-regulated phosphoprotein (DARPP-32) is a key molecule for dopamine neurotransmission. The molecular mechanisms underlying the regulation of DARPP-32 in the developing brain remains elusive. Previous studies have shown that retinoids are capable of inducing DARPP-32 in striatal cell culture, suggesting that retinoids are candidate molecules for controlling DARPP-32 expression. In the present study, we first studied the expression profiles of retinoid receptors and their associated co-factors in the developing rat telencephalon by RT-PCR. The results showed that among the retinoid receptors, RARbeta and RXRgamma were nearly selectively expressed in the developing striatum. By contrast, the retinoid receptors associated transcriptional co-factors, including the co-repressors of N-CoR and SMRT, and the co-activators of SRC-1 and P/CAF, were ubiquitously expressed in the developing telencephalon. In light of the previous findings that DARPP-32 was inducible by retinoids in striatal culture, but not in cortical culture, we hypothesized that the striatum-selective RARbeta and RXRgamma may mediate DARPP-32 induction by retinoids. To test this hypothesis, we used the gain-of-function approach to ectopically express RARbeta and RXRgamma in the developing cerebral cortex that lacked these two retinoid receptors. Ectopic expression of RARbeta1, but not RXRgamma1, up-regulated DARPP-32 in the cortical explant culture. Notably, DARPP-32 was up-regulated only by the RARbeta1 isoform, but not by other RARbeta isoforms. Our study suggests that RARbeta signaling may regulate DARPP-32 gene expression by an isoform-specific mechanism in developing telencephalic neurons. The molecular diversity of RARbeta isoforms may underlie parts of the complex gene regulation by retinoids during neural development. |
| Relation | European Journal of Neuroscience,21(12),3262-3268 |
| Type | article |
| dc.contributor | 神科所 | en_US |
| dc.creator (作者) | 廖文霖 | zh_TW |
| dc.creator (作者) | Liao, Wen-Lin ; Liu, Fu-Chin | en_US |
| dc.date (日期) | 2005.01 | en_US |
| dc.date.accessioned | 23-Sep-2014 12:27:02 (UTC+8) | - |
| dc.date.available | 23-Sep-2014 12:27:02 (UTC+8) | - |
| dc.date.issued (上傳時間) | 23-Sep-2014 12:27:02 (UTC+8) | - |
| dc.identifier.uri (URI) | http://nccur.lib.nccu.edu.tw/handle/140.119/70096 | - |
| dc.description.abstract (摘要) | Dopamine and adenosine 3`:5`-monophosphate-regulated phosphoprotein (DARPP-32) is a key molecule for dopamine neurotransmission. The molecular mechanisms underlying the regulation of DARPP-32 in the developing brain remains elusive. Previous studies have shown that retinoids are capable of inducing DARPP-32 in striatal cell culture, suggesting that retinoids are candidate molecules for controlling DARPP-32 expression. In the present study, we first studied the expression profiles of retinoid receptors and their associated co-factors in the developing rat telencephalon by RT-PCR. The results showed that among the retinoid receptors, RARbeta and RXRgamma were nearly selectively expressed in the developing striatum. By contrast, the retinoid receptors associated transcriptional co-factors, including the co-repressors of N-CoR and SMRT, and the co-activators of SRC-1 and P/CAF, were ubiquitously expressed in the developing telencephalon. In light of the previous findings that DARPP-32 was inducible by retinoids in striatal culture, but not in cortical culture, we hypothesized that the striatum-selective RARbeta and RXRgamma may mediate DARPP-32 induction by retinoids. To test this hypothesis, we used the gain-of-function approach to ectopically express RARbeta and RXRgamma in the developing cerebral cortex that lacked these two retinoid receptors. Ectopic expression of RARbeta1, but not RXRgamma1, up-regulated DARPP-32 in the cortical explant culture. Notably, DARPP-32 was up-regulated only by the RARbeta1 isoform, but not by other RARbeta isoforms. Our study suggests that RARbeta signaling may regulate DARPP-32 gene expression by an isoform-specific mechanism in developing telencephalic neurons. The molecular diversity of RARbeta isoforms may underlie parts of the complex gene regulation by retinoids during neural development. | en_US |
| dc.format.extent | 339758 bytes | - |
| dc.format.mimetype | application/pdf | - |
| dc.language.iso | en_US | - |
| dc.relation (關聯) | European Journal of Neuroscience,21(12),3262-3268 | en_US |
| dc.subject (關鍵詞) | dopamine;retinoic acid;RXR;striatum | en_US |
| dc.title (題名) | RARbeta isoform-specific regulation of DARPP-32 gene expression: An ectopic expression study in the developing telencephalon | en_US |
| dc.type (資料類型) | article | en |