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題名 神經激胺在記憶上所扮演的角色
作者 陳美如
貢獻者 李小媛
陳美如
日期 1989
1988
上傳時間 3-May-2016 14:17:22 (UTC+8)
摘要 摘要
     過去研究發現median raphe 與dorsal raphe在解剖分化與功能反應上諸多不同，而5-hydroxytryptamine在記憶上扮演抑制或促進的角色，研究結果則不一致。本研究主要目的是以passive avoidance task為模式，探討5-hydroxytryptamine在記憶鞏固上所扮演的角色及mesolimbic serotonergic pathway 與mesostriatal serotonergic pathway在記憶上是否有分化現象。
     本研究採實驗法。實驗動物為雄性Sprague-Dawley種的大白鼠102隻。實驗分三部份進行，第一部份：側腦室；第二部份：海馬迴；第三部份：紋狀體，動物被隨機分派至控制組(注射ascorbic acid) 和實驗組(注射5，7-dihydroxytryptamine) 然後，測量控制組和實驗組在記憶上的表現及腦中5-hydroxytryptamine 與norepinephrine的差異。
     實驗結果採單因子變異數分析及曼─惠特尼U考驗。結果顯示：(l)記憶測量方面：在前腦和海馬迴實驗組與控制組沒達到顯著差異，而在紋狀體實驗組的表現明顯的高於控制組(2)化學分析方面：在前腦、海馬迴和紋狀體，實驗組的5-hydroxytryptamine明顯的低於控制組而兩組在norepinephrine上的差異均未達到顯著水準。其原因可能是5-hydroxytryptamine破壞程度不同，或則是5-hydroxytryptamine在腦中不同區域扮演不同的角色所致。
參考文獻 參考文獻
     一、中文部份
     1.林清山 心理與教育統計學 台北 東華書局 民62年。
     二、英文部分
     1. Archer Trevor (1982) Serotonin and fear retention in the rats. Journal of Comparative Psychology, 96(3), 491-516.
     2. Dahlstrom. A. and K. Fuxe. (1974) Evidence for the existence of monoamine-containing neurons in the central nervous system. Acta Physiol. Scand, 62: Supple. 232, 1-55,
     3. Essman, W.B. (19~3) Neuromolecular modulation of experimentally induedretrograde ammesia. Confinia neurology, 35, 1-22.
     4. Fibiger, H. C., Lepiane, F. G. and Phillips, A.G. (1987) Disruption of memory produced by stimulation of the dorsal raphe nucleus: mediation by serotonin. Brain Research. ISS. 380-386.
     5. Fuxe, K. (1965) "Evidence for the existence of monoamine neurons in the control nervous svstem." IV. Distribution of monoamine nerve terminals nervous in central svstem." Acta Physiol. Scand.64, Supple, 247, 37-85.
     6. Gekson, S. and Baldessarini, R.F. (1975) Selective destruction of serotonin terminals In rat forebrain by high dose of 5, 7-dihydroxytryptamine. Brain Research, 85, 140-145.
     7. Geyer, M.A. and Lee, E.H.Y. (1984) Effects of clonidine, piperoxane and locus coeruleus lesion on the serotonergic and dopaminergic system in raphe and caudate nucleus. Biochemical Pharmacology, 3399-3404.
     8. Geyer, M.A., Flicker, C.E. and Lee, E.H . Y. (1982) Effect of tactile startle on serotonin content of midbrain raphe neurons in rats. Behavioral Brain Research, 4, 369-376.
     9. Geyer, M.A., Puerto, Dawsey, W.J., Wilson, S.K, Ballard, p. and Mandel I .AJ. (1976) Histologic and enzymatic studies of the mesolimbic and mesosrriaral serotonergic pathways, Brain Research, 106, 241-256.
     10. Geyer, M.A., Puerto, Menkes, D.B.,Segal ,D.S and Mandell, A.J. (1976) Behavioral studies following lesions of the mesolimbic and mesostriatal serotonergic pathways. Brain Research, 106, 25 7-270.
     11. Geyer, M.A, Dawsey, W.J. and Mandell, A. J . (1975) Differential effect of caffeine, d-amphetamine and methylphenidate on individual raphe cell fluorescence: a microspectrofluorimetric demonstration. Brain Research, 85, 135-139.
     12. Jacobs, B., William and Taylar, K.M. (1974) Defferential behavioral and neurochemical effects fllowing lesion s of the dorsal or median. Brain Research, 79. 353-361.
     13. Lalonde, R. and V. Vikis-Freibergs (1982) Manipulation s of 5-HT activity and memory in the rat. Pharmacology Biochemistry & Behavior, 22, 377-382.
     14. Lee, E.H.Y. (1989) Role of septal 5-HT In memory formation in rat. In preparation.
     15. Lee. E.H.Y. (1989) Intra-hippocampal CRF injection Improve memory retention in rat. In preparation.
     16. Lee, E.H. Y., Lin.H.H and Yin.H.M. (1987) Differential influences of different stressors upon midbrain raphe neurons in rats. Neuroscience, 80, 115-119.
     17. Lee, E.H.Y., Wang. F.B., Tang,Y.P. and Geyer, M .A. (1987) Gabaergic interneurons in the dorsal raphe mediate the effects of apomorphine on sertonergic system. Brain Research Bulletin, 18. 345-353
     18. LeeE. H.Y. (1986) Additive effects of apomorphine and colonidine on serotonin neurons in the dorsal raphe. Life Sciences, 40, 635-542.
     19. Lee, E.M.Y and Geyer, M.A. (1984) Indirect effects of apomorphine on serotonergic neurons in rats. Neuroscience, 11(2), 437-442.
     20. Lee, E.H.Y and Geyer, M.A. (1982) Selective effects of apomorphine on dorsal raphe neurons: a cytofluorimetric studv. Brain Rese arch Bulletin, 9, 719-725.
     21. Liang, K.C. and Lee, E.H.Y. (1988) Intra-amygdala injection of corticotropin releasing factor facilitate inhibitory avoidance learning and reduce exploratory behavior in rats. Psychopharmacology, 96, 232-236.
     22. O"gren, S.O., Fuse, K., Archer. T. Hall, H., Holm, A.C. and Kohlor, C. (1981)
     Studies on the role of central 5-HT neurons in avoidance learning: a behavioral and biochemical in serotonin: Current aspects of neurochemistry and function. edited by B. H aber. S. Gabay. M. R. Issidorides and S. G. A. Alivisatos New York Plenum Press, 681-705.
     23. O"gren, S.O. (1982) Forebrain serotonin and avoidance learning: behavioral and biochemistry. Studies on one-wav active avoidance learning in male rat. Pharmacology Biochemistry & Behavioral, 16, 881-895.
     24. Peat. M.A .and Gibb, J, W. (1983) High-Performance liquid chromatographic determination of indoleamines, dopamine, and norepinephrine in rat brain with fluorometric detection. Analytical Biochemistry, 128. 275-280.
     25. Ponzio, F. and Jonsson, G. (1978) Effects of neonatal 5, 7-dihydroxytryptamine treatment on the development of serotonin neurons and their transmitter metabolism. Developmental neuroscience, 1, 80-89.
     26. Wetzell, W, Getsova, V.M., Jork, R. and Matthies, H (1979) Effect of serotonin on Y-maze retention and hippocampal protein synthesis in rats. Pharmacology Biochemistry & Behavior, 12, 319-322.
描述 碩士
國立政治大學
心理學系
資料來源 http://thesis.lib.nccu.edu.tw/record/#B2002005534
資料類型 thesis
dc.contributor.advisor 李小媛zh_TW
dc.contributor.author (Authors) 陳美如zh_TW
dc.creator (作者) 陳美如zh_TW
dc.date (日期) 1989en_US
dc.date (日期) 1988en_US
dc.date.accessioned 3-May-2016 14:17:22 (UTC+8)-
dc.date.available 3-May-2016 14:17:22 (UTC+8)-
dc.date.issued (上傳時間) 3-May-2016 14:17:22 (UTC+8)-
dc.identifier (Other Identifiers) B2002005534en_US
dc.identifier.uri (URI) http://nccur.lib.nccu.edu.tw/handle/140.119/90182-
dc.description (描述) 碩士zh_TW
dc.description (描述) 國立政治大學zh_TW
dc.description (描述) 心理學系zh_TW
dc.description.abstract (摘要) 摘要
     過去研究發現median raphe 與dorsal raphe在解剖分化與功能反應上諸多不同，而5-hydroxytryptamine在記憶上扮演抑制或促進的角色，研究結果則不一致。本研究主要目的是以passive avoidance task為模式，探討5-hydroxytryptamine在記憶鞏固上所扮演的角色及mesolimbic serotonergic pathway 與mesostriatal serotonergic pathway在記憶上是否有分化現象。
     本研究採實驗法。實驗動物為雄性Sprague-Dawley種的大白鼠102隻。實驗分三部份進行，第一部份：側腦室；第二部份：海馬迴；第三部份：紋狀體，動物被隨機分派至控制組(注射ascorbic acid) 和實驗組(注射5，7-dihydroxytryptamine) 然後，測量控制組和實驗組在記憶上的表現及腦中5-hydroxytryptamine 與norepinephrine的差異。
     實驗結果採單因子變異數分析及曼─惠特尼U考驗。結果顯示：(l)記憶測量方面：在前腦和海馬迴實驗組與控制組沒達到顯著差異，而在紋狀體實驗組的表現明顯的高於控制組(2)化學分析方面：在前腦、海馬迴和紋狀體，實驗組的5-hydroxytryptamine明顯的低於控制組而兩組在norepinephrine上的差異均未達到顯著水準。其原因可能是5-hydroxytryptamine破壞程度不同，或則是5-hydroxytryptamine在腦中不同區域扮演不同的角色所致。		zh_TW
dc.description.tableofcontents 目錄
     序言
     論文摘要
     第一章 導論
     第一節 文獻探討………l
     第二節 研究目的………5
     第二章 研究方法
     第一節 實驗方法………7
     第二節 手術………7
     第三節 藥品………8
     第四節 儀器設備………9
     第五節 實驗程序………19
     第六節 組織切片………20
     第七節 統計分析………20
     第三章 實驗結果
     第一節 組織切片檢驗解剖位置………21
     第二節 one-way passive avoidance task記憶測量………21
     第三節 生化分析檢驗5-HT的破壞程度………27
     第四節 5-HT在腦中各區域的破壞程度比較………27
     第四章 討論………33
     第五章 結果與建議………35
     參考文獻………36
     圖表目錄
     圖一: 對在標準的HPLC螢光測定生化分析圖………10
     圍二: 前腦的HPLC螢光測定生化分析圖……… 11
     圖三: 海馬迴的HPLC螢光測定生化分析圖………12
     圖四: 紋狀體竹的HPLC螢光測定生化分析圖………13
     圖五: 腹側縫合核的HPLC螢光測定生化分析圖………14
     圖六: 背側縫合核的HPLC螢光測定生化分析間………15
     圓七: 海馬迴解剖圖顯示出染劑注射在dentate gyrus的位置………22
     圖八: 紋狀體解剖圖顯示出染劑注射在dentate gyrus 的位置………23
     圖九: 大白鼠側腦室注射ascorbic acid或5，7-DHT對其記憶表現的影響………24
     圍十: 大白鼠海馬迴注射ascorbic acid或5，7-DHT對其記憶表現的影響………25
     圖十一: 大白鼠紋狀體注射ascrobicacid 或5，7-DHT對其記憶表現的影響………26
     表一: 大白鼠側腦室注射5，7-DHT，對其前腦5-HT和NE濃度的影響………28
     表二: 大白鼠海馬迴注射5，7-DHT，對其海馬迴和腹側縫合核5-HT濃度的影響………29
     表三: 大白鼠海馬迴注射5，7-DHT，對其海馬迴和腹側縫合核NE濃度的影響………31
     表四: 大白鼠紋狀體注射5，7-DHT，對其紋狀體和背側縫合核5-HT濃度的影響………32		zh_TW
dc.source.uri (資料來源) http://thesis.lib.nccu.edu.tw/record/#B2002005534en_US
dc.title (題名) 神經激胺在記憶上所扮演的角色zh_TW
dc.type (資料類型) thesisen_US
dc.relation.reference (參考文獻) 參考文獻
     一、中文部份
     1.林清山 心理與教育統計學 台北 東華書局 民62年。
     二、英文部分
     1. Archer Trevor (1982) Serotonin and fear retention in the rats. Journal of Comparative Psychology, 96(3), 491-516.
     2. Dahlstrom. A. and K. Fuxe. (1974) Evidence for the existence of monoamine-containing neurons in the central nervous system. Acta Physiol. Scand, 62: Supple. 232, 1-55,
     3. Essman, W.B. (19~3) Neuromolecular modulation of experimentally induedretrograde ammesia. Confinia neurology, 35, 1-22.
     4. Fibiger, H. C., Lepiane, F. G. and Phillips, A.G. (1987) Disruption of memory produced by stimulation of the dorsal raphe nucleus: mediation by serotonin. Brain Research. ISS. 380-386.
     5. Fuxe, K. (1965) "Evidence for the existence of monoamine neurons in the control nervous svstem." IV. Distribution of monoamine nerve terminals nervous in central svstem." Acta Physiol. Scand.64, Supple, 247, 37-85.
     6. Gekson, S. and Baldessarini, R.F. (1975) Selective destruction of serotonin terminals In rat forebrain by high dose of 5, 7-dihydroxytryptamine. Brain Research, 85, 140-145.
     7. Geyer, M.A. and Lee, E.H.Y. (1984) Effects of clonidine, piperoxane and locus coeruleus lesion on the serotonergic and dopaminergic system in raphe and caudate nucleus. Biochemical Pharmacology, 3399-3404.
     8. Geyer, M.A., Flicker, C.E. and Lee, E.H . Y. (1982) Effect of tactile startle on serotonin content of midbrain raphe neurons in rats. Behavioral Brain Research, 4, 369-376.
     9. Geyer, M.A., Puerto, Dawsey, W.J., Wilson, S.K, Ballard, p. and Mandel I .AJ. (1976) Histologic and enzymatic studies of the mesolimbic and mesosrriaral serotonergic pathways, Brain Research, 106, 241-256.
     10. Geyer, M.A., Puerto, Menkes, D.B.,Segal ,D.S and Mandell, A.J. (1976) Behavioral studies following lesions of the mesolimbic and mesostriatal serotonergic pathways. Brain Research, 106, 25 7-270.
     11. Geyer, M.A, Dawsey, W.J. and Mandell, A. J . (1975) Differential effect of caffeine, d-amphetamine and methylphenidate on individual raphe cell fluorescence: a microspectrofluorimetric demonstration. Brain Research, 85, 135-139.
     12. Jacobs, B., William and Taylar, K.M. (1974) Defferential behavioral and neurochemical effects fllowing lesion s of the dorsal or median. Brain Research, 79. 353-361.
     13. Lalonde, R. and V. Vikis-Freibergs (1982) Manipulation s of 5-HT activity and memory in the rat. Pharmacology Biochemistry & Behavior, 22, 377-382.
     14. Lee, E.H.Y. (1989) Role of septal 5-HT In memory formation in rat. In preparation.
     15. Lee. E.H.Y. (1989) Intra-hippocampal CRF injection Improve memory retention in rat. In preparation.
     16. Lee, E.H. Y., Lin.H.H and Yin.H.M. (1987) Differential influences of different stressors upon midbrain raphe neurons in rats. Neuroscience, 80, 115-119.
     17. Lee, E.H.Y., Wang. F.B., Tang,Y.P. and Geyer, M .A. (1987) Gabaergic interneurons in the dorsal raphe mediate the effects of apomorphine on sertonergic system. Brain Research Bulletin, 18. 345-353
     18. LeeE. H.Y. (1986) Additive effects of apomorphine and colonidine on serotonin neurons in the dorsal raphe. Life Sciences, 40, 635-542.
     19. Lee, E.M.Y and Geyer, M.A. (1984) Indirect effects of apomorphine on serotonergic neurons in rats. Neuroscience, 11(2), 437-442.
     20. Lee, E.H.Y and Geyer, M.A. (1982) Selective effects of apomorphine on dorsal raphe neurons: a cytofluorimetric studv. Brain Rese arch Bulletin, 9, 719-725.
     21. Liang, K.C. and Lee, E.H.Y. (1988) Intra-amygdala injection of corticotropin releasing factor facilitate inhibitory avoidance learning and reduce exploratory behavior in rats. Psychopharmacology, 96, 232-236.
     22. O"gren, S.O., Fuse, K., Archer. T. Hall, H., Holm, A.C. and Kohlor, C. (1981)
     Studies on the role of central 5-HT neurons in avoidance learning: a behavioral and biochemical in serotonin: Current aspects of neurochemistry and function. edited by B. H aber. S. Gabay. M. R. Issidorides and S. G. A. Alivisatos New York Plenum Press, 681-705.
     23. O"gren, S.O. (1982) Forebrain serotonin and avoidance learning: behavioral and biochemistry. Studies on one-wav active avoidance learning in male rat. Pharmacology Biochemistry & Behavioral, 16, 881-895.
     24. Peat. M.A .and Gibb, J, W. (1983) High-Performance liquid chromatographic determination of indoleamines, dopamine, and norepinephrine in rat brain with fluorometric detection. Analytical Biochemistry, 128. 275-280.
     25. Ponzio, F. and Jonsson, G. (1978) Effects of neonatal 5, 7-dihydroxytryptamine treatment on the development of serotonin neurons and their transmitter metabolism. Developmental neuroscience, 1, 80-89.
     26. Wetzell, W, Getsova, V.M., Jork, R. and Matthies, H (1979) Effect of serotonin on Y-maze retention and hippocampal protein synthesis in rats. Pharmacology Biochemistry & Behavior, 12, 319-322.		zh_TW