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題名 水丁香抗老化作用之研究
Studies on the anti-aging effects of Ludwigia octovalvis
作者 陳俊伊
貢獻者 王培育
陳俊伊
關鍵詞 水丁香
卡路里節制
壽命
氧化壓力
類胰島素生長因子的訊號傳導
日期 2011
上傳時間 30-Oct-2012 11:28:33 (UTC+8)
摘要 水丁香在台灣已被廣泛用作為香料和中草藥。目前已經被證實擁有許多生物活性，例如抗氧化、抗肝毒性、抗菌及抗癌等。在本研究中，我們利用兩種不同果蠅株w1118和Canton-S(CS)餵食兩種不同的飲食條件，分別為標準食物配方(15% yeast和15% dextrose)及卡路里節制(calorie restriction, CR)食物配方(5% yeast和5% dextrose)，來評估水丁香水萃取物對果蠅老化的影響。我們測試不同水丁香水萃取物濃度(0.1% 及0.5％)的食物對於果蠅的壽命、食物攝取量、下蛋率、活動能力、學習記憶、氧化壓力和基因表現等影響。我們發現水丁香水萃取物在標準食物配方下對於兩種品系的母果蠅均有延長壽命的表現，但並不影響果蠅的下蛋率及活動能力，另外水丁香水萃取物明顯的延緩了老化過程中學習能力退化的情況。然而於CR食物中添加水丁香水萃取物，其延長果蠅壽命的現象則消失，顯示水丁香水萃取物延長果蠅壽命的現象可能透過卡路里節制相關路徑。水丁香水萃取物可增加果蠅抵抗paraquat所引發的氧化壓力，並且降低抗氧化基因如superoxide dismutase(sod1及sod2)、catalase以及果蠅類胰島素胜肽(Drosophila insulin-like peptide, dilp2及dilp3) mRNA的表現。
     綜合以上實驗結果我們推測，水丁香水萃取物延長壽命可能的作用機制為：(1)透過卡路里節制的路徑。 (2)影響類胰島素生長因子的訊號傳導(Insulin/IGF-1 signaling)。(3)增加抗氧化壓力的能力。
Ludwigia octovalvis (Jacq.) P. H. Raven has been widely used as a spice and herbal medicine in Taiwan. It has been proved to possess many biological activities, antioxidative, anti-hepatotoxic, anti-bacterial and anticancer properties, for example. In this research, Ludwigia octovalvis extract (LOE) was used in two different diet conditions, one is standard diet (15% yeast and 15% dextrose), and the other is calorie restriction (5% yeast and 5% dextrose). The effect of LOE on the lifespan of the flies was estimated using w1118 and Canton-S strains of Drosophila melanogaster. We also evaluated the effects of 0.1% or 0.5% of LOE on food intake, fecundity, locomotor activity, cognitive performance, anti-oxidative stress and age-related gene expression in flies under standard diet. Our results showed that LOE could extend the lifespan of female flies on both species without affecting the fecundity and locomotor activity. Intriguingly, LOE attenuated the cognitive decline in both male and female flies. The longevity effect of LOE, however, was vanished when the lifespan of the flies was examined under CR diet, suggesting that LOE may extend lifespan through a CR-related pathway. LOE also increased resistance of paraquat-induced oxidative stress and down regulated the mRNA expression of sod1, sod2, cat, dilp2 and dilp3 in the flies.
     In summary, our results indicated that LOE may extend lifespan through: (1) a CR-related pathway. (2) regulation of Insulin/IGF-1 signaling. (3) increase of anti-oxidative stress.
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描述 碩士
國立政治大學
神經科學研究所
98754007
100
資料來源 http://thesis.lib.nccu.edu.tw/record/#G0098754007
資料類型 thesis
dc.contributor.advisor 王培育zh_TW
dc.contributor.author (Authors) 陳俊伊zh_TW
dc.creator (作者) 陳俊伊zh_TW
dc.date (日期) 2011en_US
dc.date.accessioned 30-Oct-2012 11:28:33 (UTC+8)-
dc.date.available 30-Oct-2012 11:28:33 (UTC+8)-
dc.date.issued (上傳時間) 30-Oct-2012 11:28:33 (UTC+8)-
dc.identifier (Other Identifiers) G0098754007en_US
dc.identifier.uri (URI) http://nccur.lib.nccu.edu.tw/handle/140.119/54654-
dc.description (描述) 碩士zh_TW
dc.description (描述) 國立政治大學zh_TW
dc.description (描述) 神經科學研究所zh_TW
dc.description (描述) 98754007zh_TW
dc.description (描述) 100zh_TW
dc.description.abstract (摘要) 水丁香在台灣已被廣泛用作為香料和中草藥。目前已經被證實擁有許多生物活性，例如抗氧化、抗肝毒性、抗菌及抗癌等。在本研究中，我們利用兩種不同果蠅株w1118和Canton-S(CS)餵食兩種不同的飲食條件，分別為標準食物配方(15% yeast和15% dextrose)及卡路里節制(calorie restriction, CR)食物配方(5% yeast和5% dextrose)，來評估水丁香水萃取物對果蠅老化的影響。我們測試不同水丁香水萃取物濃度(0.1% 及0.5％)的食物對於果蠅的壽命、食物攝取量、下蛋率、活動能力、學習記憶、氧化壓力和基因表現等影響。我們發現水丁香水萃取物在標準食物配方下對於兩種品系的母果蠅均有延長壽命的表現，但並不影響果蠅的下蛋率及活動能力，另外水丁香水萃取物明顯的延緩了老化過程中學習能力退化的情況。然而於CR食物中添加水丁香水萃取物，其延長果蠅壽命的現象則消失，顯示水丁香水萃取物延長果蠅壽命的現象可能透過卡路里節制相關路徑。水丁香水萃取物可增加果蠅抵抗paraquat所引發的氧化壓力，並且降低抗氧化基因如superoxide dismutase(sod1及sod2)、catalase以及果蠅類胰島素胜肽(Drosophila insulin-like peptide, dilp2及dilp3) mRNA的表現。
     綜合以上實驗結果我們推測，水丁香水萃取物延長壽命可能的作用機制為：(1)透過卡路里節制的路徑。 (2)影響類胰島素生長因子的訊號傳導(Insulin/IGF-1 signaling)。(3)增加抗氧化壓力的能力。		zh_TW
dc.description.abstract (摘要) Ludwigia octovalvis (Jacq.) P. H. Raven has been widely used as a spice and herbal medicine in Taiwan. It has been proved to possess many biological activities, antioxidative, anti-hepatotoxic, anti-bacterial and anticancer properties, for example. In this research, Ludwigia octovalvis extract (LOE) was used in two different diet conditions, one is standard diet (15% yeast and 15% dextrose), and the other is calorie restriction (5% yeast and 5% dextrose). The effect of LOE on the lifespan of the flies was estimated using w1118 and Canton-S strains of Drosophila melanogaster. We also evaluated the effects of 0.1% or 0.5% of LOE on food intake, fecundity, locomotor activity, cognitive performance, anti-oxidative stress and age-related gene expression in flies under standard diet. Our results showed that LOE could extend the lifespan of female flies on both species without affecting the fecundity and locomotor activity. Intriguingly, LOE attenuated the cognitive decline in both male and female flies. The longevity effect of LOE, however, was vanished when the lifespan of the flies was examined under CR diet, suggesting that LOE may extend lifespan through a CR-related pathway. LOE also increased resistance of paraquat-induced oxidative stress and down regulated the mRNA expression of sod1, sod2, cat, dilp2 and dilp3 in the flies.
     In summary, our results indicated that LOE may extend lifespan through: (1) a CR-related pathway. (2) regulation of Insulin/IGF-1 signaling. (3) increase of anti-oxidative stress.		en_US
dc.description.tableofcontents 中文摘要.............................................. I
     英文摘要.............................................. II
     目錄 .................................................III
     圖次 ..................................................VI
     表次 ................................................ VII
     縮寫對照表 ........................................... VIII
     中英文名詞對照表 ...................................... IX
     第一章 緒論 .......................................... 01
     第一節、 水丁香簡介 ................................... 01
     第二節、 老化的理論 ................................... 02
     一、 卡路里節制(CR)與老化關聯 .......................... 03
     二、 氧化壓力(oxidative stress)與老化關聯 .............. 04
     三、 基因與壽命的相關性 ................................ 05
     (一) 抗氧化酵素(antioxidant enzymes) .................. 05
     (二) 類胰島素生長因子的訊號傳導(insulin/IGF-1 signaling) 06
     第三節、 老化相關指標 ................................... 06
     一、 生殖能力(fecundity) ............................... 06
     二、 自發性活動能力(spontaneous motor activity) ......... 07
     三、 老化的記憶損害AMI（age-related memory impairment） ...07
     第四節、 利用果蠅模式之藥物篩選研究的優處 .................. 08
     第五節、 實驗設計 ....................................... 08
     第二章 實驗材料與研究方法 ................................ 09
     第一節、 實驗材料 ....................................... 09
     一、 實驗藥品 ........................................... 09
     二、 果蠅種類 ........................................... 10
     三、 食物培養基配方 ...................................... 10
     第二節、 研究方法 ........................................ 10
     一、 水丁香水抽出物之製備 ................................. 10
     二、 果蠅飼養與存活率分析試驗 ...............................11
     三、抗氧化壓力試驗(paraquat) .............................. 11
     四、 食物攝取量試驗 ....................................... 11
     五、 生殖能力試驗 ......................................... 12
     六、 自發性活動能力試驗 .................................... 12
     七、 學習能力試驗 ......................................... 12
     八、 mRNA之定量分析 ....................................... 13
     (一) RNA之萃取 ........................................... 13
     (二) DNase treatment .................................... 14
     (三) First strand cDNA synthesis ........................ 14
     (四) 即時定量聚合酶連鎖反應(quantitative PCR)............... 15
     九、 統計分析 ............................................ 16
     第三章 結果 .............................................. 17
     第一節、 餵食水丁香水萃取物對於w1118果蠅壽命的影響 ........... 17
     第二節、 餵食水丁香水萃取物對於CS果蠅壽命的影響 .............. 20
     第三節、 餵食水丁香水萃取物對於w1118果蠅食物攝取量的分析 ...... 23
     第四節、 餵食水丁香水萃取物對於w1118果蠅下蛋率的影響 ......... 25
     
     第五節、 餵食水丁香水萃取物對於w1118果蠅自發性活動能力的影響 .. 27
     第六節、 餵食水丁香水萃取物對於w1118果蠅學習能力的影響 ....... 29
     第七節、 在CR狀態餵食水丁香水萃取物對於w1118果蠅壽命的影響 ... 31
     第八節、 餵食水丁香水萃取物對於w1118果蠅抗氧化能力試驗分析 ... 34
     第九節、 餵食水丁香水萃取物對於w1118果蠅Insulin/IGF-1 signaling及抗氧化基因表現的分析 .................................... 37
     第四章 討論 .............................................. 39
     第五章 結論 .............................................. 44
     參考文獻 ................................................. 45
     附錄圖一、水丁香全株之外觀 ................................. XI
     附錄圖二、抗氧化防禦系統示意圖 ............................ XII
     附錄圖三、酵母菌至哺乳類動物，insulin/IGF-1 signaling與壽命之間訊息調控模式 . ............................................XIII
     附錄圖四、實驗流程圖 ..................................... XIV
     附錄圖五、水丁香水萃示意流程圖 ............................ XV
     附錄圖六、果蠅活動能力偵測儀器 ............................. V
     附錄圖七、t-maze儀器 ................................... XVII
     附錄表一、水丁香所含七大分類與十三種化合物個別之生物活性表 .. XVIII		zh_TW
dc.language.iso en_US-
dc.source.uri (資料來源) http://thesis.lib.nccu.edu.tw/record/#G0098754007en_US
dc.subject (關鍵詞) 水丁香zh_TW
dc.subject (關鍵詞) 卡路里節制zh_TW
dc.subject (關鍵詞) 壽命zh_TW
dc.subject (關鍵詞) 氧化壓力zh_TW
dc.subject (關鍵詞) 類胰島素生長因子的訊號傳導zh_TW
dc.title (題名) 水丁香抗老化作用之研究zh_TW
dc.title (題名) Studies on the anti-aging effects of Ludwigia octovalvisen_US
dc.type (資料類型) thesisen
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