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題名 A gene delivery system for insect cells mediated by arginine-rich cell-penetrating peptides
作者 Chen, Yung-Jen ; Liu, Betty Revon ; Dai, Yun-Hao ; Lee, Cheng-Yi ; Chan, Ming-Huan ; Chen, Hwei-Hsien ; Chiang, Huey-Jenn ; Lee, Han-Jung
詹銘煥
貢獻者 神科所
關鍵詞 Cell-penetrating peptide (CPP); Cellular internalization; Polyarginine; Protein transduction; Spodoptera frugiperda; Transgenic insects
日期 2012.02
上傳時間 9-Apr-2014 17:18:18 (UTC+8)
摘要 Most bioactive macromolecules, such as protein, DNA and RNA, basically cannot permeate into cells freely from outside the plasma membrane. Cell-penetrating peptides (CPPs) are a group of short peptides that possess the ability to traverse the cell membrane and have been considered as candidates for mediating gene and drug delivery into living cells. In this study, we demonstrate that three arginine-rich CPPs (SR9, HR9 and PR9) are able to form stable complexes with plasmid DNA and deliver DNA into insect Sf9 cells in a noncovalent manner. The transferred plasmid DNA containing enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) coding regions could be expressed in cells functionally assayed at both the protein and RNA levels. Furthermore, treatment of cells with CPPs and CPP/DNA complexes resulted in a viability of 84–93% indicating these CPPs are not cytotoxic. These results suggest that arginine-rich CPPs appear to be a promising tool for insect transgenesis
關聯 Gene,493(2), 201-210
資料來源 http://dx.doi.org/10.1016/j.gene.2011.11.060
資料類型 article
dc.contributor 神科所en_US
dc.creator (作者) Chen, Yung-Jen ; Liu, Betty Revon ; Dai, Yun-Hao ; Lee, Cheng-Yi ; Chan, Ming-Huan ; Chen, Hwei-Hsien ; Chiang, Huey-Jenn ; Lee, Han-Jungen_US
dc.creator (作者) 詹銘煥zh_TW
dc.date (日期) 2012.02en_US
dc.date.accessioned 9-Apr-2014 17:18:18 (UTC+8)-
dc.date.available 9-Apr-2014 17:18:18 (UTC+8)-
dc.date.issued (上傳時間) 9-Apr-2014 17:18:18 (UTC+8)-
dc.identifier.uri (URI) http://nccur.lib.nccu.edu.tw/handle/140.119/65233-
dc.description.abstract (摘要) Most bioactive macromolecules, such as protein, DNA and RNA, basically cannot permeate into cells freely from outside the plasma membrane. Cell-penetrating peptides (CPPs) are a group of short peptides that possess the ability to traverse the cell membrane and have been considered as candidates for mediating gene and drug delivery into living cells. In this study, we demonstrate that three arginine-rich CPPs (SR9, HR9 and PR9) are able to form stable complexes with plasmid DNA and deliver DNA into insect Sf9 cells in a noncovalent manner. The transferred plasmid DNA containing enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) coding regions could be expressed in cells functionally assayed at both the protein and RNA levels. Furthermore, treatment of cells with CPPs and CPP/DNA complexes resulted in a viability of 84–93% indicating these CPPs are not cytotoxic. These results suggest that arginine-rich CPPs appear to be a promising tool for insect transgenesisen_US
dc.format.extent 2144074 bytes-
dc.format.mimetype application/pdf-
dc.language.iso en_US-
dc.relation (關聯) Gene,493(2), 201-210en_US
dc.source.uri (資料來源) http://dx.doi.org/10.1016/j.gene.2011.11.060en_US
dc.subject (關鍵詞) Cell-penetrating peptide (CPP); Cellular internalization; Polyarginine; Protein transduction; Spodoptera frugiperda; Transgenic insectsen_US
dc.title (題名) A gene delivery system for insect cells mediated by arginine-rich cell-penetrating peptidesen_US
dc.type (資料類型) articleen